CT Imaging Biomarkers Predict Clinical Outcomes After Pancreatic Cancer Surgery

This study aimed to determine whether changes in contrast-enhanced computed tomography (CT) parameters could predict postsurgery overall and progression-free survival (PFS) in pancreatic cancer patients. Seventy-nine patients with a final pathological diagnosis of pancreatic adenocarcinoma were included in this study from June 2008 to August 2012. Dynamic contrast-enhanced (DCE) CT of tumors was obtained before curative-intent surgery. Absolute enhancement change (AEC) and relative enhancement change (REC) were evaluated on DCE-CT. PFS and overall survival (OS) were compared based on CT enhancement patterns. The markers of fibrogenic alpha-smooth muscle antigen (α-SMA) and periostin in tumor specimens were evaluated by immunohistochemical staining. The χ² test was performed to determine whether CT enhancement patterns were associated with α-SMA-periostin expression levels (recorded as positive or negative). Lower REC (<0.9) was associated with shorter PFS (HR 0.51, 95% CI: 0.31–0.89) and OS (HR 0.44, 95% CI: 0.25–0.78). The α-SMA and periostin expression level were negatively correlated with REC (both P = 0). Among several CT enhancement parameters, REC was the best predictor of patient postsurgery survival. Low REC was associated with a short progression-free time and poor survival. The pathological studies suggested that REC might be a reflection of cancer fibrogenic potential.     

全髋关节置换术治疗髋臼骨折初期治疗失败病例的中期随访结果

背景背景:髋臼骨折常见于高能量的暴力损伤,即使经过初期有效治疗仍可能在后期出现髋关节骨关节炎、股骨头缺血性坏死以及坐骨神经损伤等并发症而需要进一步治疗。目的:回顾性分析全髋关节置换术(THA)治疗髋臼骨折初期治疗失败病例的中期临床和影像学随访结果。方法方法:1998年9月至2011年8月行THA治疗髋臼骨折初期治疗失败患者108例(110髋),其中96例(98髋)获得随访,随访时间26~146个月,平均54.2个月。比较其中51例既往采用切开复位内固定治疗和47例既往采用保守治疗患者所行THA的疗效差异。采用Harris髋关节评分系统进行临床评价,依据术后X线片进行影像学分析。结果结果:髋关节Harris评分从术前平均46.3分(14~78分)提高至末次随访的平均91.4分(56~100分)(P<0.001)。并发症包括术中股骨矩骨折4例、脱位1例、医源性坐骨神经损伤4例、假体周围感染2例及BrookerⅢ级异位骨化3例。仅有1例患者因全骨水泥型假体无菌性松动行翻修术。结论结论:采用THA治疗髋臼骨折初期治疗失败病例的中期临床和影像学结果较为满意,具有较高的假体生存率和患者满意度,但在技术上存在诸多难点,围手术期管理和手术细节等方面需要特别注意。     

长鞘联合大球囊阻断技术在破裂腹主动脉瘤腔内治疗应用

目的 评价长鞘联合大球囊阻断技术在破裂腹主动脉瘤腔内治疗的效果及其治疗经验.方法 回顾性分析新疆维吾尔自治区人民医院血管外科2009年2月-2014年6月共11例破裂腹主动脉瘤患者的临床资料.其中男性9例,女性2例;年龄69 ～ 88岁,平均76岁.动脉粥样硬化性腹主动脉瘤7例,腹主动脉假性动脉瘤2例,感染性腹主动脉瘤2例.11例术前均完成CT动脉造影检查,急诊行腔内修复术,术中使用长鞘联合大球囊阻断技术.结果 术中无患者死亡.术后1例患者出现造影剂肾病;1例出现腹腔间隔室综合征,均在1周后死亡.随访期间,1例出现Ⅰ型内漏,再次行腔内治疗;1例假性动脉瘤复发再次破裂,放弃治疗.结论 长鞘联合大球囊阻断技术在破裂腹主动脉瘤腔内治疗是安全有效的治疗方式.     

Body mass index,waist‐to‐hip ratio,waist circumference and waist‐to‐height ratio cannot predict male semen quality: a report of 1231 subfertile Chinese men

There were controversial results between obesity‐associated markers and semen quality. In this study, we investigated the correlations between age, obesity‐associated markers including body mass index (BMI), waist‐to‐hip ratio (WHR), waist‐to‐height ratio (WHtR) and waist circumference (WC), the combination of age and obesity‐associated markers, semen parameters and serum reproductive hormone levels in 1231 subfertile men. The results showed that BMI, WC, WHR and WHtR were positively related to age, and there were also positive relations between BMI, WHR, WC and WHtR and between sperm concentration (SC), total sperm count (TSC), progressive motility (PR), sperm motility and per cent of normal sperm morphology (NSM). However, age, each of obesity‐associated markers and the combination of obesity‐associated markers and age were unrelated to any of semen parameters including total normal‐progressively motile sperm count (TNPMS). Age, BMI, WHR, WC and WHtR were negatively related to serum testosterone and SHBG levels. However, only serum LH and FSH levels were negatively related to sperm concentration, NSM and sperm motility. In a conclusion, although age and obesity have significant impacts on reproductive hormones such as testosterone, SHBG and oestradiol, semen parameters related to FSH and LH could not be influenced, indicating that obesity‐associated markers could not predict male semen quality.     

基于KTQ的人文关怀护理在CCU病房中的实践

目的总结基于KTQ的人文关怀护理在CCU病房中的实践经验。方法将80例患者按入住时间分为常规护理组和人文关怀组各40例。常规护理组按常规实施护理,人文关怀组运用KTQ的管理模式,找出引起CCU患者焦虑的主要问题,实施人文关怀护理。结果通过人文关怀护理的实施,CCU患者焦虑明显下降,舒适度提高,护士满意度提高。结论基于KTQ管理模式的人文关怀对最大程度地减少CCU患者焦虑和不安心理有着重要意义。     

Alterations of gene profiles in Leydig-cell-regenerating adult rat testis after ethane dimethane sulfonate-treatment

Only occupying about 1%–5% of total testicular cells, the adult Leydig cell (ALC) is a unique endocrine cell that produces androgens. Rat Leydig cells regenerate after these cells in the testis are eliminated with ethane dimethane sulfonate (EDS). In this study, we have characterized Leydig cell regeneration and messenger ribonucleic acids (mRNA) profiles of EDS treated rat testes. Serum testosterone, testicular gene profiling and some steroidogenesis-related proteins were analyzed at 7, 21, 35 and 90 days after EDS treatment. Testicular testosterone levels declined to undetectable levels until 7 days after treatment and then started to recover. Seven days after treatment, 81 mRNAs were down-regulated greater than or equal to two-fold, with 48 becoming undetectable. These genes increased their expression 21 days and completely returned to normal levels 90 days after treatment. The undetectable genes include steroidogenic pathway proteins: steroidogenic acute regulatory protein, Scarb1, Cyp11a1, Cyp17a1, Hsd3b1, Cyp1b1 and Cyp2a1. Seven days after treatment, there were 89 mRNAs up-regulated two-fold or more including Pkib. These up-regulated mRNAs returned to normal 90 days after treatment. Cyp2a1 did not start to recover until 35 days after treatment, indicating that this gene is only expressed in ALCs not in the precursor cells. Quantitative polymerase chain reaction, western blotting and semi-quantitative immunohistochemical staining using tissue array confirmed the changes of several randomly picked genes and their proteins.     

The reference values for semen parameters of 1213 fertile men in Guangdong Province in China

Semen samples were collected from 1213 fertile men whose partners had a time-to-pregnancy (TTP) ≤12 months in Guangdong Province in Southern China, and semen parameters including semen volume, sperm concentration, total counts, motility, and morphology were evaluated according to the World Health Organization (WHO) 2010 guideline. All semen parameters analyzed were normal in ~62.2% of the total samples, whereas ~37.8% showed at least one of the semen parameters below normal threshold values. The fifth centiles (with 95% confidence intervals) were 1.3 (1.2–1.5) ml for semen volume, 20 × 10⁶ (18×10⁶–20×10⁶) ml⁻¹ for sperm concentration, 40 × 10⁶ (38×10⁶–44×10⁶) per ejaculate for total sperm counts, 48% (47%–53%) for vitality, 39% (36%–43%) for total motility, 25% (23%–27%) for sperm progressive motility, 5.0% (4%–5%) for normal morphology. The pH values ranged from 7.2 to 8.0 with the mean ± standard deviation at 7.32 ± 0.17. No effects of age and body mass index were found on semen parameters. Occupation, smoking and alcohol abuse, varicocele appeared to decrease semen quality. Sperm concentration, but not sperm morphology, is positively correlated with TTP, whereas vitality is negatively correlated with TTP. Our study provides the latest reference values for the semen parameters of Chinese fertile men in Guangdong Province, which are close to those described in the new WHO guidelines (5^th Edition).     

口腔颌面部非何杰金氏淋巴瘤流式细胞DNA分析及意义探讨

目的:探讨流式细胞学各项指标与非何杰金氏淋巴瘤(NHL)发病部位、临床分期、病理分级、细胞类型及预后的关系和流式细胞学对NHL的诊断价值。方法:采用流式细胞仪测定50例口腔颌面部NHL及10例反应性增生淋巴结(RLN)石蜡包埋组织的DNA倍体、细胞周期各参数,并分析各参数及其与预后的关系。结果:10例RLN皆为二倍体,而NHL二倍体率为54%,异倍体率为46%,二者有显著性差异。SPF值、S+G2/M值与非何杰金氏淋巴瘤恶性程度密切相关,SPF值、S+G2/M值随肿瘤的恶性程度增大而增大。结论:口腔颌面部淋巴瘤流式细胞学检测具有一定诊断学价值,但其运用一定要与形态学相结合。同时SPF、S+G2/M等指标可以反映其细胞增殖状况及病理学恶性程度。     

Correlation between levels of fibrinogen, beta455 g/A fibrinogen gene polymorphism and chronic periodontitis]

OBJECTIVE: To investigate the relationship between plasma levels of fibrinogen, the-beta455 G/A fibrinogen gene polymorphism and the severity of periodontal inflammation and to explore the possible role of fibrinogen in the association of periodontitis with coronary heart disease (CHD). METHODS: A total of 121 patients with moderate to severe periodontitis and periodontally healthy and gingivitis controls were enrolled in the study. Peripheral blood samples were collected and the plasma fibrinogen levels were determined by the clotting method of Clauss. Polymerase chain reaction and restriction fragment length polymorphism analysis with Hae III were used to examine the -beta455 G/A fibrinogen gene polymorphism. RESULTS: Fibrinogen levels were significantly higher in moderately or severely chronic periodontitis patients [(3.45 +/- 0.68) g/L] than periodontally healthy and gingivitis controls [(2.47 +/- 0.42) g/L, P < 0.001]. The carrier status of the A allele at position -455 in the beta fibrinogen gene was associated with elevated fibrinogen levels and the frequency of the-A455 allele in the beta fibrinogen gene in the patient group was significantly higher than in the control group (P = 0.032). Carriers of the -A455 allele were about 3-fold more likely to have moderate or severe periodontitis as compare to individuals without the -A455 allele( OR = 3. =135, P= 0.008). CONCLUSIONS: Fg-beta455 G/A polymorphism may contribute to the elevated plasma fibrinogen levels and put individuals at higher risk of having severe periodontitis. As the independent risk factor of CHD, fibrinogen levels and Fg-beta455 G/A polymorphism may play a role in the pathogenesis of periodontitis.     

PBL的病案准备

PBL是一种以学生为中心,以问题为导向,围绕病例进行的讨论式的全新教学模式。病案作为学生学习的焦点、讨论的框架在PBL的教学中起着至关重要的作用。本文从PBL教案的作用、使用、特点和撰写步骤等几个方面具体阐述了如何为PBL准备一个好的病案。